점액표피양암종세포주에서 Bax 활성화 유도를 통한 Cisplatin의 항암효능
Bax Activation as Therapeutic Strategy with Cisplatin in Mucoepidermoid Carcinoma Cell lines
유현주, 조남표, 정요셉, 신지애,
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유현주 ( Yu Hyun-Ju ) - 전북대학교 치의학전문대학원 구강병리학교실
조남표 ( Cho Nam-Pyo ) - 전북대학교 치의학전문대학원 구강병리학교실
정요셉 ( Jeong Joseph H. ) - 서울대학교 수의과대학 수의발생학교실
신지애 ( Shin Ji-Ae ) - 전북대학교 치의학전문대학원 구강생체과학연구소
KMID : 0829220160400050839
Abstract
Background: Cisplatin is a well-known platinum-containing anti-cancer drug against bladder, ovarian, lung and testicular cancer. However, the potential effects and molecular targets of cisplatin in human mucoepidermoid carcinoma (MEC) are not fully understood. Here, we investigated the apoptotic effect and underlying mechanism of cisplatin in human MEC cells.
Methods: The potential effects of cisplatin were evaluated by trypan blue exclusion assay, Western blotting, 4’-6-diamidino-2-phenylindole (DAPI) staining, live/dead assay and immunocytochemistry.
Results: Cisplatin suppressed cell growth and enhanced expression of cleaved PAPR in MC3 and YD15 cells. Cisplatin caused morphological change of nuclei and increased the number of ethidium homodimer-1-stained cells. In addition, cisplatin commonly increased Bax activation in both cells, while other Bcl-2 family proteins were not affected.
Conclusions: These results suggest that cisplatin might induce apoptosis by activating Bax protein, which would provide baseline data for development of effective treatment strategy against MEC.
키워드
Cisplatin; Mucoepidermoid carcinoma; Bax activation; Apoptosis
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